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OncoKids: A Comprehensive Next-Generation Sequencing Panel for Pediatric Malignancies.
Matthew C Hiemenz1, Dejerianne G Ostrow2, Tracy M Busse2
1Department of Pathology and Laboratory Medicine, Children's Hospital Los Angeles, Los Angeles, California; Department of Pathology, Keck School of Medicine of USC, Los Angeles, California.
The Journal of Molecular Diagnostics : JMD
|August 24, 2018
Summary
The OncoKids panel is a new next-generation sequencing assay for pediatric cancers. It accurately detects key genetic markers in various sample types, supporting its use in routine clinical testing.
Area of Science:
- Oncology
- Genetics
- Molecular Diagnostics
Background:
- Pediatric malignancies require accurate diagnostic, prognostic, and therapeutic markers.
- Next-generation sequencing (NGS) offers a comprehensive approach to identify these markers.
- Existing molecular assays may have limitations in scope or sample compatibility for pediatric cancers.
Purpose of the Study:
- To validate the OncoKids panel, an NGS assay for comprehensive molecular profiling of pediatric tumors.
- To assess the panel's performance using a diverse set of clinical samples.
- To determine the suitability of the OncoKids panel for routine clinical application in pediatric oncology.
Main Methods:
- The OncoKids panel utilizes amplification-based NGS for DNA and RNA analysis.
- It targets cancer predisposition loci, tumor suppressor genes, oncogenes, mutation hotspots, amplification events, and gene fusions.
- Validation involved 192 unique clinical samples (formalin-fixed, paraffin-embedded, frozen tissue, bone marrow, peripheral blood) with various pediatric tumor types and alterations.
Main Results:
- The panel demonstrated robust analytical sensitivity, reproducibility, and limit of detection.
- It successfully detected diagnostic, prognostic, and therapeutic markers across a wide spectrum of pediatric malignancies.
- Performance was consistent across different sample types and tumor histologies.
Conclusions:
- The OncoKids panel is a validated NGS assay for the molecular characterization of pediatric cancers.
- Its comprehensive target content and compatibility with various sample types make it suitable for clinical use.
- The panel supports routine clinical testing for a broad range of pediatric malignancies, aiding in personalized treatment strategies.
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