Using Nesprin Tension Sensors to Measure Force on the LINC Complex

Mechanotransduction, or the process by which mechanical forces regulate cellular functions, is increasingly studied in a variety of different physiological and pathological contexts. Although these forces are most often studied at cell-matrix and cell-cell adhesions, recent work has shown that the nuclear LINC complex is also subject to mechanical forces. Here we describe how to use a FRET-based biosensor, known as TSmod, in the LINC complex protein nesprin-2G. This approach allows for measurement of LINC complex forces in living cells with spatial-temporal resolution.