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Updated: Feb 6, 2026

Expression, Detergent Solubilization, and Purification of a Membrane Transporter, the MexB Multidrug Resistance Protein
Published on: December 3, 2010
LmrP from Lactoccoccus lactis: a tractable model to understand secondary multidrug transport in MFS
Aurélie Roth1, Cedric Govaerts1
1SFMB, Université Libre de Bruxelles (ULB) CP206/02, Boulevard du Triomphe, Building BC, B-1050 Brussels, Belgium.
Abstract:
The secondary transporter LmrP from Lactoccoccus lactis is a remarkable model to study the molecular basis of secondary multidrug transport. This review article addresses more than twenty years of research about transport activity, substrates range, conformational dynamics and mechanistic models of drug export for LmrP. Several studies have shown that the transporter alternates between inward-open and outward-open conformations and that the transition is regulated by the protonation state of key acidic residues and is further modulated by the lipid environment.
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