Effect of thermal trauma on numbers and function of T and B cells from mouse spleen

Swiss-Webster female mice were given a moderately severe burn, and studies were carried out on the number and function of T and B cells from the spleens of burned and normal mice. The results showed a significant decrease (p less than 0.05) in the number of T and B cells for 2-3 days after burning with a rapid return to normal and a subsequent rise above normal at 14 and 21 days postburn (p less than 0.05). In the test for function, burned mice had a significant decrease in spontaneous mitotic activity of both T and B cells during the 21-day postburn period. When spleen lymphocytes were incubated with purified mitogens, both T and B cells showed a significantly diminished mitotic response in most of the burned animals.