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A Fast and Simple Qualitative Method for Screening Oleaginous Yeasts on Agar
Xochitl Niehus1, Leticia Casas-Godoy2, Marcos Vargas-Sánchez1
1Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C. (CIATEJ), 800 Normalistas Av., 44270 Guadalajara, JAL, Mexico.
Journal of Lipids
|August 29, 2018
Summary
A new Rhodamine B dye method rapidly screens oleaginous yeasts for lipid production on agar plates. This economical approach identifies high-lipid-producing yeast strains using alternative carbon sources.
Area of Science:
- Microbiology
- Biotechnology
- Biochemistry
Background:
- Oleaginous yeasts are crucial for producing microbial oils, but current screening methods are slow and labor-intensive.
- Efficient identification of lipid-producing yeast strains is essential for various industrial applications.
Purpose of the Study:
- To develop a novel, rapid, cost-effective, and simple qualitative method for screening oleaginous yeasts.
- To utilize the fluorescent properties of Rhodamine B for direct correlation with yeast lipid content.
Main Methods:
- A new method using Rhodamine B dye incorporated into agar plates for yeast cultivation.
- Visual observation under visible light to distinguish colored oleaginous yeasts from uncolored non-oleaginous ones.
- Adaptation of the method for alternative carbon sources like lignocellulosic materials and glycerol.
Main Results:
- The Rhodamine B method effectively differentiates oleaginous yeasts based on color development correlated to lipid accumulation.
- Screening of 124 yeast isolates on glucose, glycerol, and agave bagasse hydrolysate identified five strains producing over 48% lipids.
- The assay was successfully adapted for screening both nonpigmented and reddish-pigmented yeast strains.
Conclusions:
- The developed Rhodamine B agar plate method offers a fast, economical, and versatile tool for screening oleaginous yeasts.
- This method facilitates the discovery of high-performing yeast strains for microbial oil production, even with alternative feedstocks.
- The technique has broad applicability in microbial oil research and industrial biotechnology.
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