Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Introduction to Membrane Proteins01:16

Introduction to Membrane Proteins

81.3K
The cell membrane, or plasma membrane, is an ever-changing landscape. It is described as a fluid mosaic where various macromolecules are embedded in the phospholipid bilayer. Among the macromolecules are proteins. The protein content varies across cell types. For example, mitochondrial inner membranes contain ~76% protein content, while myelin contains ~18% protein content. Individual cells contain many types of membrane proteins—red blood cells contain over 50—and different cell...
81.3K
Formal Charges02:42

Formal Charges

40.6K
In some cases, there are seemingly more than one valid Lewis structures for molecules and polyatomic ions. The concept of formal charges can be used to help predict the most appropriate Lewis structure when more than one reasonable structure exists.
40.6K
Ions and Ionic Charges03:27

Ions and Ionic Charges

79.2K
In ordinary chemical reactions, the nucleus — which contains the protons and neutrons of each atom and thus identifies the element — remains unchanged. Electrons, however, can be added to atoms by transfer from other atoms, lost by transfer to other atoms, or shared with other atoms. The transfer and sharing of electrons among atoms govern the chemistry of the elements. During the formation of some compounds, atoms gain or lose electrons to form electrically charged particles called...
79.2K
The Resting Membrane Potential01:21

The Resting Membrane Potential

142.9K
Overview
142.9K
Membrane Proteins01:30

Membrane Proteins

30.5K
Plasma membranes have integral transmembrane proteins involved in facilitated transport. These proteins are collectively referred to as transport proteins, and they function as either channels for the material or as carriers themselves. Channel proteins have hydrophilic domains exposed to the intracellular and extracellular fluids and a hydrophilic channel through their core that provides a hydrated opening for solutes to pass through the membrane layers. Passage through the channel allows...
30.5K
Protein Diffusion in the Membrane01:24

Protein Diffusion in the Membrane

5.7K
Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...
5.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Immunoaffinity-free chromatographic purification of ovarian cancer biomarker CA125 (MUC16) from blood serum enables mass spectrometry characterization.

Analytical methods : advancing methods and applications·2024
Same author

Hydrolysis of Whey Protein-Dextran Glycates Made Using the Maillard Reaction.

Foods (Basel, Switzerland)·2019
Same author

Kinetics of Whey Protein Glycation Using Dextran and the Dry-Heating Method.

Foods (Basel, Switzerland)·2019
Same author

Fractionation of Glycomacropeptide from Whey Using Positively Charged Ultrafiltration Membranes.

Foods (Basel, Switzerland)·2018
Same author

Ultrafiltration behavior of partially retained proteins and completely retained proteins using equally-staged single pass tangential flow filtration membranes.

Biotechnology progress·2018
Same author

Adsorbed Layer Thickness Determination for Convective-Based Media from Pressure Drop Data.

Analytical chemistry·2018
Same journal

The Potential for Bioactive Peptide Production in a Fermented Dairy Beverage Based on Chickpea Water Extract Using Proteolytic Lactic Acid Bacteria.

Foods (Basel, Switzerland)·2026
Same journal

Influence of Protein Concentration on Heat-Induced Fouling of Oat Drink.

Foods (Basel, Switzerland)·2026
Same journal

Microalgae as Future Foods: Unlocking Their Potential and Overcoming Barriers to Market Adoption and Commercialization.

Foods (Basel, Switzerland)·2026
Same journal

Effect of High-Intensity Ultrasound and Calcium Chelation on Functional Properties of Casein Micelles.

Foods (Basel, Switzerland)·2026
Same journal

GC-MS and GC-IMS Based Metabolomics Combined with Cellular Assays to Characterize Volatile Compounds and Pharmacological Activity of <i>Lysimachia foenum-graecum</i> Hance from Different Origins.

Foods (Basel, Switzerland)·2026
Same journal

Research on the Potential Mechanism of Guanine Nucleotides Enhancing the Tolerance of <i>Lactiplantibacillus plantarum</i> Y12.

Foods (Basel, Switzerland)·2026
See all related articles

Related Experiment Video

Updated: Feb 6, 2026

Concentration of Virus Particles from Environmental Water and Wastewater Samples Using Skimmed Milk Flocculation and Ultrafiltration
10:53

Concentration of Virus Particles from Environmental Water and Wastewater Samples Using Skimmed Milk Flocculation and Ultrafiltration

Published on: March 17, 2023

2.4K

Milk Protein Concentration Using Negatively Charged Ultrafiltration Membranes.

Abhiram Arunkumar1, Mark R Etzel2

  • 1Department of Chemical and Biological Engineering, University of Wisconsin, 1605 Linden Drive, Madison, WI 53706, USA. arunkumar@uwalumni.com.

Foods (Basel, Switzerland)
|August 30, 2018
PubMed
Summary
This summary is machine-generated.

This study introduces novel wide-pore charged membranes for milk protein concentrate (MPC) production. These membranes significantly enhance permeate flux and protein recovery compared to traditional methods, optimizing dairy processing.

Keywords:
caseindairydeposit layerpermeatepolyethersulfoneregenerated celluloseretentatesieving coefficient

More Related Videos

Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli
08:46

Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli

Published on: January 6, 2015

33.6K
Milk Collection in the Rat Using Capillary Tubes and Estimation of Milk Fat Content by Creamatocrit
07:38

Milk Collection in the Rat Using Capillary Tubes and Estimation of Milk Fat Content by Creamatocrit

Published on: December 16, 2015

13.1K

Related Experiment Videos

Last Updated: Feb 6, 2026

Concentration of Virus Particles from Environmental Water and Wastewater Samples Using Skimmed Milk Flocculation and Ultrafiltration
10:53

Concentration of Virus Particles from Environmental Water and Wastewater Samples Using Skimmed Milk Flocculation and Ultrafiltration

Published on: March 17, 2023

2.4K
Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli
08:46

Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli

Published on: January 6, 2015

33.6K
Milk Collection in the Rat Using Capillary Tubes and Estimation of Milk Fat Content by Creamatocrit
07:38

Milk Collection in the Rat Using Capillary Tubes and Estimation of Milk Fat Content by Creamatocrit

Published on: December 16, 2015

13.1K

Area of Science:

  • Dairy Science
  • Membrane Technology
  • Food Engineering

Background:

  • Milk protein concentrate (MPC) production typically uses unmodified, size-exclusion based ultrafiltration membranes.
  • Current methods face limitations in flux and efficiency for concentrating skim milk.

Purpose of the Study:

  • To investigate the efficacy of wide-pore, negatively charged ultrafiltration membranes for MPC manufacturing.
  • To compare the performance of charged membranes against conventional unmodified membranes in skim milk processing.

Main Methods:

  • Utilized wide-pore, negatively charged 100 kDa ultrafiltration membranes for skim milk concentration and diafiltration.
  • Mimicked industrial MPC production processes using tangential-flow filtration.
  • Developed and applied mass balance models to analyze concentration and diafiltration steps.

Main Results:

  • Charged membranes demonstrated at least a four-fold higher permeate flux compared to unmodified 30 kDa membranes at equivalent protein recovery.
  • Electrostatic repulsion, not just size exclusion, was identified as the primary mechanism for protein rejection.
  • Mass balance models accurately predicted experimental outcomes for both concentration and diafiltration.

Conclusions:

  • Wide-pore charged membranes offer a significant advancement in MPC manufacturing efficiency.
  • This technology enhances protein recovery and permeate flux through electrostatic interactions.
  • The developed mass balance model provides a valuable tool for optimizing charged membrane processes in the dairy industry.