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The Effect of the Application of Thyme Essential Oil on Microbial Load During Meat Drying
Published on: March 14, 2018
[Study on Subcritical Fluid Extraction of Essential Oil from Cinnamomum camphora and Its Antibacterial Activity]
Objective:
To determine the optimum extract condition of essential oil from dry Cinnamomum camphora leaves and to study its antibacterial activity.
Methods:
The essential oil was extracted by subcritical fluid, and analyzed by response surface methodology based on single factor test. The chemical compositions of essential oil were analyzed by GC-MS, and the components were quantitatively determined by normalization method. The agar disc diffusion and dilution broth method were used to determine antibacterial activity.
Results:
The extraction rate was highest as 3. 54%,which matched with the predictive yield of 3. 56%,and the extraction time was30 min, the extraction temperature was 40 ℃,and resolution temperature was 65 ℃. 47 chemical constituents were identified and isolated from Cinnamomum camphora, which was analyzed by GC-MS. The main chemical constituents were eucalyptol( 24. 74%),bicyclo[3. 1. 0]hexan-4-methylene-1-( 1-methylethyl) ( 7. 05%),linalool( 5. 82%),caryophyllene ( 4. 75%). The essential oil from Cinnamomum camphora had different degrees of inhibition on Escherichia coli,Staphylococcus aureus, Pseudomonas aeruginosa,Bacillus subtilis.
Conclusion:
Subcritical fluid is suitable for the extraction of essential oil from Cinnamomum camphora and the essential oil has a good antimicrobial activity.
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