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OsPIN1b is Involved in Rice Seminal Root Elongation by Regulating Root Apical Meristem Activity in Response to Low
Huwei Sun1,2, Jinyuan Tao1, Yang Bi1
1State Key Laboratory of Crop Genetics and Germplasm Enhancement, and Key Laboratory of Plant Nutrition and Fertilization in Low-Middle Reaches of the Yangtze River, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, 210095, China.
Scientific Reports
|August 31, 2018
Summary
Rice root growth under nutrient deficiency is regulated by OsPIN1b, a key auxin transporter. This protein mediates the effects of nitric oxide and strigolactones, crucial for root development in low-nitrogen and low-phosphate conditions.
Area of Science:
- Plant Biology
- Molecular Biology
- Agricultural Science
Background:
- Plant root development is crucial for crop production and is significantly impacted by nutrient availability.
- Auxin, nitric oxide (NO), and strigolactones (SLs) are known regulators of root growth, particularly under nutrient-deficient conditions.
- The specific roles of PIN genes, essential for polar auxin transport, in nutrient-modulated root growth are not fully understood.
Purpose of the Study:
- To investigate the role of specific OsPIN genes in rice root development under low-nitrogen (LN) and low-phosphate (LP) conditions.
- To elucidate the involvement of OsPIN1b in mediating the effects of NO and SLs on root growth and meristem activity.
Main Methods:
- Quantitative real-time PCR (qRT-PCR) to analyze OsPIN gene expression.
- Phenotypic analysis of seminal roots in ospin1b mutants and wild-type rice.
- Measurement of [3H]IAA transport and IAA concentration.
- Reporter gene assays (pCYCB1;1::GUS) to assess meristem activity.
Main Results:
- OsPIN1b expression is highest in the root tip and is downregulated by LN, LP, NO donor (SNP), and SL analogue (GR24).
- The ospin1b mutant exhibited shorter seminal roots and attenuated responses to LN, LP, SNP, and GR24 in terms of root growth, auxin transport, and IAA levels.
- Meristem activity, indicated by pCYCB1;1::GUS expression, was upregulated by LN, LP, SNP, and GR24 in wild-type but not in the ospin1b mutant.
Conclusions:
- OsPIN1b plays a significant role in rice root development under low-nitrogen and low-phosphate stress.
- OsPIN1b functions as a critical mediator of auxin transport, downstream of NO and SL signaling pathways.
- OsPIN1b is essential for maintaining meristem activity in the root tip under nutrient-deficient conditions.
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