Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mechanism of Breathing I: Inspiration01:30

Mechanism of Breathing I: Inspiration

3.3K
Introduction to Inspiration: The Respiratory System in Action
The respiratory system, an essential network for breathing, comprises the conducting and respiratory zones, each playing a crucial role in the overall process of respiration. Let us explore the detailed mechanism of inspiration, or inhalation, which is the first phase of the respiratory cycle.
Pathway of Air during Inspiration
During inspiration, air enters our body through the nose or mouth and moves through the conducting zone,...
3.3K
Microtubule Associated Proteins (MAPs)01:42

Microtubule Associated Proteins (MAPs)

5.9K
Microtubule function and architecture are regulated by an array of specialized proteins called microtubule-associated proteins or MAPs. These proteins are widespread across different organisms and have conserved protein motifs, like the multi-TOG domain for tubulin binding found in the CLASP family of MAPs. Some MAPs are lineage-specific based on their conserved domains. Their functions depend upon the cytoskeletal architecture and cell type they are located within. In-plant cells, a specific...
5.9K
Statistical Software for Data Analysis and Clinical Trials01:12

Statistical Software for Data Analysis and Clinical Trials

1.6K
Statistical software is pivotal in data analysis and clinical trials by providing tools to analyze data, draw conclusions, and make predictions. These software packages range from simple data management applications to complex analytical platforms, supporting various statistical tests, models, and simulation techniques. Their significance lies in their ability to handle vast amounts of data with precision and efficiency, enabling researchers to validate hypotheses, identify trends, and make...
1.6K
GIS Software, Hardware, and Sources of GIS Data01:23

GIS Software, Hardware, and Sources of GIS Data

795
A Geographic Information System (GIS) combines specialized software and hardware to effectively manage, analyze, and present spatial and related data. GIS software includes critical functionalities such as a user interface for easy navigation, database management tools for handling spatial and attribute data, and data retrieval features for efficient access. Analytical tools transform raw data into insights, while display functions produce maps and reports in various formats for effective...
795
Plotting of Topographic Maps01:29

Plotting of Topographic Maps

534
Topographic maps represent the Earth's surface features using contour lines, which connect points of equal elevation to create a two-dimensional representation of three-dimensional terrain. Creating a topographic map requires a systematic approach.Begin by plotting a scaled grid and marking intersections corresponding to the survey's elevation data points. Assign elevation values at these intersections to build the base map. Next, determine contour levels using a consistent contour interval,...
534
Coordinates and Map Projections01:29

Coordinates and Map Projections

620
Coordinates and map projections are essential tools in accurately representing the Earth's surface for various applications, ranging from navigation to spatial analysis. The latitude and longitude coordinate system is a universally recognized framework for defining locations. Latitude specifies the distance of a point north or south of the equator, measured in degrees from 0° at the equator to 90° at the poles. Longitude indicates a location's position east or west of the prime meridian,...
620

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Gaussian Mixture Model-Based Focused Refinement for Enhanced Flexible Structure Determination in CryoEM and CryoET.

ArXiv·2026
Same author

Structural dynamics of kappa opioid receptor interactions with β-arrestin 1.

Nature communications·2026
Same author

C-terminal dimerization motifs control asynchronous chain elongation during modular polyketide biosynthesis.

The Journal of biological chemistry·2026
Same author

Human NLRP3 inflammasome activation leads to formation of condensate at the microtubule organizing center.

Science advances·2026
Same author

Candesartan cilexetil disrupts methicillin-resistant Staphylococcus aureus membrane and potentiates gentamicin and polymyxin B activity.

Nature communications·2026
Same author

Mechanism of Gating and Isoform-Specific Inhibition in Renal CLC Chloride Channels.

bioRxiv : the preprint server for biology·2026
Same journal

Ultrastructural evidence of autophagy-related processes and mitochondrial remodeling in the myxozoan parasite Henneguya piaractus.

Journal of structural biology·2026
Same journal

Architecture and dynamics of a supramolecular oxygen transport system in human homogentisate 1,2-Dioxygenase.

Journal of structural biology·2026
Same journal

Connecting pathways between mineralized fibrocartilage and bone at the Achilles tendon insertion.

Journal of structural biology·2026
Same journal

Structural and functional characterization of thermostable EstS1 esterase for BHET degradation.

Journal of structural biology·2026
Same journal

Following the white rabbit: multiscale 2D3D correlative imaging of bone structure.

Journal of structural biology·2026
Same journal

The mantis shrimp eye imaged in 3D using 4th generation synchrotron multiscale phase contrast tomography.

Journal of structural biology·2026
See all related articles

Related Experiment Video

Updated: Feb 5, 2026

MRI Mapping of Cerebrovascular Reactivity via Gas Inhalation Challenges
09:33

MRI Mapping of Cerebrovascular Reactivity via Gas Inhalation Challenges

Published on: December 17, 2014

14.5K

New software tools in EMAN2 inspired by EMDatabank map challenge.

James M Bell1, Muyuan Chen2, Tunay Durmaz2

  • 1Graduate Program in Quantitative and Computational Biology, Baylor College of Medicine, United States.

Journal of Structural Biology
|September 7, 2018
PubMed
Summary
This summary is machine-generated.

Recent updates to the EMAN2 software suite enhance cryo-electron microscopy (cryo-EM) data processing. New tools improve particle picking, quality assessment, and 3D reconstruction filtering for higher resolution cryo-EM maps.

Keywords:
3-D reconstructionCryoEMEMDatabank map challengeImage processingSingle particle analysisStructural biology

More Related Videos

Integrating Remote Sensing with Species Distribution Models; Mapping Tamarisk Invasions Using the Software for Assisted Habitat Modeling SAHM
12:26

Integrating Remote Sensing with Species Distribution Models; Mapping Tamarisk Invasions Using the Software for Assisted Habitat Modeling SAHM

Published on: October 11, 2016

13.8K
Use of Three-Dimensional Imaging Reconstruction Software as a Training Tool for Cranial Vena Cava Venipuncture in the Ferret
04:18

Use of Three-Dimensional Imaging Reconstruction Software as a Training Tool for Cranial Vena Cava Venipuncture in the Ferret

Published on: July 15, 2025

1.1K

Related Experiment Videos

Last Updated: Feb 5, 2026

MRI Mapping of Cerebrovascular Reactivity via Gas Inhalation Challenges
09:33

MRI Mapping of Cerebrovascular Reactivity via Gas Inhalation Challenges

Published on: December 17, 2014

14.5K
Integrating Remote Sensing with Species Distribution Models; Mapping Tamarisk Invasions Using the Software for Assisted Habitat Modeling SAHM
12:26

Integrating Remote Sensing with Species Distribution Models; Mapping Tamarisk Invasions Using the Software for Assisted Habitat Modeling SAHM

Published on: October 11, 2016

13.8K
Use of Three-Dimensional Imaging Reconstruction Software as a Training Tool for Cranial Vena Cava Venipuncture in the Ferret
04:18

Use of Three-Dimensional Imaging Reconstruction Software as a Training Tool for Cranial Vena Cava Venipuncture in the Ferret

Published on: July 15, 2025

1.1K

Area of Science:

  • Structural Biology
  • Biophysics
  • Computational Biology

Background:

  • EMAN2 is a versatile software suite for cryo-electron microscopy (cryo-EM) data analysis.
  • Previous versions had limitations in particle picking and data processing workflows.

Purpose of the Study:

  • To significantly improve the single particle analysis (SPA) workflow within EMAN2.
  • To enhance the accuracy and efficiency of cryo-EM map generation and analysis.

Main Methods:

  • Development of a convolutional neural network (CNN) for automated particle picking.
  • Introduction of an automated particle quality metric to replace human-biased methods.
  • Modification of 3D reconstruction filtering and implementation of local resolution-based iterative filtration.

Main Results:

  • Dramatically improved particle picking accuracy, especially for challenging datasets.
  • Accurate identification of "bad" particles with minimal computational overhead.
  • Enhanced appearance of sidechains in high-resolution structures and improved local resolution in cryo-EM maps.

Conclusions:

  • The enhanced EMAN2 software suite offers a more robust and accurate cryo-EM data processing pipeline.
  • These improvements facilitate higher resolution structural determination using single particle analysis.
  • The updated tools provide significant advantages for the cryo-EM community.