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Updated: Feb 5, 2026

Corneal Donor Tissue Preparation for Descemet's Membrane Endothelial Keratoplasty
Published on: September 17, 2014
A New User-Friendly and Affordable Method in Descemet Stripping Endothelial Keratoplasty
Mohammad Soleimani1, Seyed Ali Tabatabaei1, Reza Mirshahi1
1Eye Research center, Farabi Eye Hospital, Tehran University of Medical Sciences, Tehran, Iran.
Aims:
To describe a new technique in Descemet Stripping Automated Endothelial Keratoplasty (DSAEK).
Materials And Methods:
In this technique, we use easily available materials (mainly a Nelaton tube) to make an injector for loading DSAEK lenticule and also easily pulling it (using a gauge 23 needle) into the eye. In this paper, we report outcomes of this technique in four cases.
Results:
Using these available instruments could easily lead to a clear postoperative cornea. Mean Endothelial Cell Density (ECD) loss at sixth postoperative month was 26%.
Conclusion:
We proposed a novel effective user-friendly and affordable technique to perform DSAEK.
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