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Dual Effects of Melanoma Cell-derived Factors on Bone Marrow Adipocytes Differentiation
Published on: August 23, 2018
Dual Effects of Melanoma Cell-derived Factors on Bone Marrow Adipocytes Differentiation
Juan Wang1, Jin Wen2, Xiao-Xiang Chen3
1Department of Rheumatology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University.
Abstract:
The crosstalk between bone marrow adipocytes and tumor cells may play a critical role in the process of bone metastasis. A variety of methods are available for studying the significant crosstalk; however, a two-dimensional transwell system for coculture remains a classic, reliable, and easy way for this crosstalk study. Here, we present a detailed protocol that shows the coculture of bone marrow adipocytes and melanoma cells. Nevertheless, such a coculture system could not only contribute to the study of cell signal transductions of cancer cells induced by bone marrow adipocytes, but also to the future mechanistic study of bone metastasis which may reveal new therapeutic targets for bone metastasis.
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