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Updated: Feb 5, 2026

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Published on: July 25, 2025
Development of Colorimetric and Ratiometric Fluorescence Membranes for Detection of Nitrate in the Presence of
Hong Dinh Duong1, Han Lae Kim2, Jong Il Rhee3
1School of Chemical Engineering and Research Center for Biophotonics, Chonnam National University, Yong-Bong Ro 77, Gwangju 61186, Korea. zink1735@gmail.com.
Abstract:
In this study, a quantitative analysis of nitrate in aqueous solution was performed through the combination of an oxazine170 perchlorate⁻ethyl cellulose (O17-EC) membrane with aluminum-containing compounds. Aluminum of Devarda's alloy (DA) or a clay hydrotalcite (HT) was employed for the reduction of nitrate to produce ammonia, and the produced ammonia was detected by the O17-EC membrane. The method of combining the O17-EC membrane with aluminum compounds has showed a broad detection range of nitrate. That is, the DA was combined with the O17-EC membrane and showed the linear nitrate detection ranges of 1⁻10 mM and 10⁻100 mM, while the O17-EC membrane immobilized with the clay HT showed a linear detection range of 0.1⁻1 mM nitrate. The visual color transition of the nitrate-sensing membranes at different nitrate concentrations was clearly observed under sunlight or irradiation of a light-emitting diode (LED) at an excitation wavelength of 470 nm (LED470).
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