[Study on Chemical Constituents and Content Determination of Feifukang Mistura]

To identify the main chemical constituents and to determine the content in Feifukang mistura.

HPLC-MS technique was used to profile and identify the chemical constituents by comparing the retention time,MS data with the reference standard. The content determination of all the chemical constituents were carried on a HPLC system.

Five compounds were separated from Feifukang mistura,which were identified as neomangiferin,mangiferin,calycosin-7-O-glucoside,calycosin,and schizandrol A. The standard curves of them showed good linearity on the range of 2. 08 ~ 104. 0 μg/m L,2. 00 ~ 100. 0 μg/m L,2. 00 ~ 100. 0μg/m L,2. 09 ~ 104. 5 μg/m L,and 1. 98 ~ 99. 0 μg/m L,respectively. The average recoveries were all in the range of 91. 3 ~ 103. 8%.

The methods of chemical constituents identification and content determination were established,which may offer better revealing the material basis and controlling quality of Feifukang mistura.