Design of a simple paper-based colorimetric biosensor using polydiacetylene liposomes for neomycin detection

Area of Science:

• Analytical Chemistry
• Materials Science

Background:

• Neomycin detection is crucial for food safety and antibiotic residue monitoring.
• Existing methods for neomycin detection can be complex and time-consuming.

Purpose of the Study:

• To develop a simple, visual, and sensitive method for neomycin detection.
• To enhance the stability and performance of polydiacetylene (PDA) liposomes on paper-based analytical devices (μPADs).

Main Methods:

• Fabrication of a dot array μPAD using wax printing.
• Immobilization of self-signaling PDA liposomes onto the μPAD.
• Optimization of PDA liposome stability using hydrophilic reagents, particularly polyvinyl alcohol (PVA).
• Visual and fluorescence-based detection of neomycin.

Main Results:

• Polyvinyl alcohol (PVA) significantly enhanced the stability of PDA liposomes in the paper matrix.
• Enhanced liposome stability led to a sharp increase in detection sensitivity.
• Neomycin was successfully detected visually and via fluorescence measurement.
• The developed method achieved a detection limit of 1 ppm for neomycin and demonstrated selectivity against non-aminoglycoside antibiotics.

Conclusions:

• The combination of μPADs and PVA-stabilized PDA liposomes offers a convenient and sensitive platform for neomycin detection.
• This approach provides a promising tool for rapid on-site antibiotic residue analysis.