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Updated: Feb 5, 2026
09:54
Imaging Serotonergic Fibers in the Mouse Spinal Cord Using the CLARITY/CUBIC Technique
Published on: February 26, 2016
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3D imaging of PSD-95 in the mouse brain using the advanced CUBIC method
Huazheng Liang1,2,3, Hongqin Wang4, Shaoshi Wang5
1Neurology Department, Shanghai No.1 People's Hospital (in the process of affiliation), School of Medicine, Tongji University, Shanghai, 200081, China. a.liang@westernsydney.edu.au.
Molecular Brain
|September 14, 2018
Summary
This study visualizes postsynaptic density-95 (PSD-95) distribution in 3D using CUBIC tissue clarification. The 3D mapping reveals detailed PSD-95 distribution in mouse brain nuclei and novel findings in fiber bundles.
Area of Science:
- Neuroscience
- Molecular Biology
- Biochemistry
Background:
- Postsynaptic density-95 (PSD-95) is crucial for synaptic plasticity, learning, and memory.
- Previous studies on PSD-95 distribution relied on 2D sections, limiting comprehensive spatial understanding.
- Advanced imaging techniques are needed to visualize protein distribution in intact brain structures.
Purpose of the Study:
- To visualize the 3D distribution of PSD-95 in the intact mouse brain.
- To overcome the limitations of 2D section-based analyses for protein localization studies.
- To provide a comprehensive spatial map of PSD-95 in the C57BL/6 mouse brain.
Main Methods:
- Utilized the CUBIC (Clear, Unobstructed Brain/Body Imaging with a CLARITY-derived Intelligent-tissue-processing) technique for tissue clearing.
- Performed immunofluorescent staining for PSD-95 on clarified mouse brain hemispheres.
- Employed light sheet microscopy and 4D software for 3D reconstruction and analysis.
Main Results:
- Demonstrated a detailed 3D distribution map of PSD-95 across various brain nuclei.
- Confirmed previously reported PSD-95 distribution patterns in most brain nuclei.
- Observed strong PSD-95 signals in some fiber bundles, a novel finding requiring further investigation.
Conclusions:
- The CUBIC technique enables high-resolution 3D visualization of PSD-95 distribution in the intact mouse brain.
- 3D mapping provides a more comprehensive understanding of PSD-95 localization compared to traditional 2D methods.
- Novel PSD-95 localization in fiber bundles warrants further research into its functional implications.
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