Related Experiment Video
Updated: Feb 5, 2026

RNAi Screening for Host Factors Involved in Vaccinia Virus Infection using Drosophila Cells
Published on: August 25, 2010
Screening for Factors Involved in X Chromosome Inactivation Using Haploid ESCs
Asun Monfort1, Giulio Di Minin2, Anton Wutz3
1Department of Biology, Institute of Molecular Health Sciences, Swiss Federal Institute of Technology, ETH Hönggerberg, Zurich, Switzerland. asun.monfort@biol.ethz.ch.
Abstract:
Forward genetics can provide insight into molecular pathways as has been demonstrated by advances in cell biology from comprehensive genetic studies in simple organisms. Recently, techniques have become available that promise a similar potential for understanding developmental pathways in mammals. Here we describe a genetic forward screening approach for identifying factors involved in X chromosome inactivation that is based on haploid mouse embryonic stem cells. Using a genetically encoded selection system screening of large mutant pools can identify candidate silencing factors with high confidence.
Related Concept Videos
X-Inactivation
Chromosome Replication
Chromosome Structure
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...
Chromosome Structure
Lampbrush Chromosomes
LBCs are made up of two pairs of conjugating homologous chromatids. Each chromatid consists of alternatively positioned regions of condensed-inactive chromatin and loosely placed-active side loops, which can be contracted and extended. The loops...
Polytene Chromosomes

