A pH-Resolved Colorimetric Biosensor for Simultaneous Multiple Target Detection.

Area of Science:

• Analytical Chemistry
• Biosensor Technology
• Biochemistry

Background:

• Colorimetric detection is widely used but lacks multiplexing capabilities.
• Developing multi-target biosensors is crucial for comprehensive analysis.
• Existing methods often involve complex and expensive chemical modifications.

Purpose of the Study:

• To develop a novel pH-resolved colorimetric biosensor for simultaneous detection of four key toxins.
• To introduce pH as a dimension for controlling dye release and enabling sequential detection.
• To simplify biosensor fabrication by avoiding tedious chemical modifications.

Main Methods:

• Utilized allochroic dyes as signal indicators.
• Introduced pH control for sequential release of dyes and target detection.
• Employed DNA-directed self-assembly of graphene oxide and magnetic separation for fabrication.
• Developed a system for simultaneous detection of ochratoxin A (OTA), aflatoxins B1 (AFB1), fumonisin B1 (FB1), and microcystin-LR (MC-LR).

Main Results:

• Achieved simultaneous detection of four different toxins using a single platform.
• Demonstrated that target concentrations can be determined sequentially by adjusting pH.
• Fabrication process was simplified, avoiding high-cost chemical modifications.
• The biosensor proved to be simple, rapid, accurate, and low-cost.

Conclusions:

• The pH-resolved colorimetric biosensor offers a new strategy for multiplexed toxin detection.
• This approach simplifies biosensor fabrication and reduces costs.
• The developed method is suitable for rapid, accurate, and simultaneous analysis of multiple targets.