Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Transmission Electron Microscopy01:15

Transmission Electron Microscopy

7.3K
In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400...
7.3K
Graphing the Wave Function01:13

Graphing the Wave Function

3.1K
Consider the wave equation for a sinusoidal wave moving in the positive x-direction. The wave equation is a function of both position and time. From the wave equation, two different graphs can be plotted.
3.1K
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.9K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.9K
Overview of Electron Microscopy01:25

Overview of Electron Microscopy

14.7K
The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
14.7K
Scanning Electron Microscopy01:07

Scanning Electron Microscopy

5.5K
A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
5.5K
Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

5.5K
Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
5.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Visual analysis of particle behaviors to understand combustion simulations.

IEEE computer graphics and applicationsĀ·2014
Same author

Jet-hadron correlations in √[s(NN)]=200  GeV p+p and central Au+Au collisions.

Physical review lettersĀ·2014
Same author

cDNA cloning and expression characterization of serum transferrin gene from oriental weatherfish Misgurnus anguillicaudatus.

Journal of fish biologyĀ·2014
Same author

Cyclosporine A and tacrolimus combined with enteric-coated mycophenolate sodium influence the plasma mycophenolic acid concentration - a randomised controlled trial in Chinese live related donor kidney transplant recipients.

International journal of clinical practice. SupplementĀ·2014
Same author

Vibrational Excitations and Low Energy Electronic Structure of Epoxide-decorated Graphene.

The journal of physical chemistry lettersĀ·2014
Same author

Energy dependence of moments of net-proton multiplicity distributions at RHIC.

Physical review lettersĀ·2014
Same journal

Unsupervised deep image prior for sparse-view and limited-angle electron tomography.

UltramicroscopyĀ·2026
Same journal

Determination of the structure of the tertiary phase in the alloy Al<sub>10</sub>Mo<sub>10</sub>Nb<sub>10</sub>Ta<sub>10</sub>Ti<sub>30</sub>Zr<sub>30</sub> using convergent beam electron diffraction.

UltramicroscopyĀ·2026
Same journal

Predictive drift compensation of multi-frame STEM via live scan modification.

UltramicroscopyĀ·2026
Same journal

Deep PACBED: Multitask analysis of PACBED images using deep neural networks.

UltramicroscopyĀ·2026
Same journal

Guided progressive reconstructive imaging: A new quantization-based framework for low-dose, high-throughput and real-time analytical ptychography.

UltramicroscopyĀ·2026
Same journal

Brightness optimization in a 200 keV DTEM source by geometry-driven aberration suppression.

UltramicroscopyĀ·2026
See all related articles

Related Experiment Video

Updated: Feb 5, 2026

Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction
12:38

Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction

Published on: August 9, 2011

17.8K

An improved iterative wave function reconstruction algorithm in high-resolution transmission electron microscopy.

W Q Ming1, J H Chen1, Y T He1

  • 1College of Materials Science and Engineering, Centre for High Resolution Electron Microscopy, Hunan University, Changsha 410082, China.

Ultramicroscopy
|September 19, 2018
PubMed
Summary
This summary is machine-generated.

This study enhances iterative wavefunction reconstruction for atomic-resolution transmission electron microscopy. Improved image alignment and GPU acceleration significantly boost accuracy and speed.

Keywords:
Electron microscopyImagingPrecipitatesWavefunction reconstruction

More Related Videos

Micropatterning Transmission Electron Microscopy Grids to Direct Cell Positioning within Whole-Cell Cryo-Electron Tomography Workflows
09:53

Micropatterning Transmission Electron Microscopy Grids to Direct Cell Positioning within Whole-Cell Cryo-Electron Tomography Workflows

Published on: September 13, 2021

7.7K
Microwave Assisted Rapid Diagnosis of Plant Virus Diseases by Transmission Electron Microscopy
09:20

Microwave Assisted Rapid Diagnosis of Plant Virus Diseases by Transmission Electron Microscopy

Published on: October 14, 2011

14.1K

Related Experiment Videos

Last Updated: Feb 5, 2026

Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction
12:38

Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction

Published on: August 9, 2011

17.8K
Micropatterning Transmission Electron Microscopy Grids to Direct Cell Positioning within Whole-Cell Cryo-Electron Tomography Workflows
09:53

Micropatterning Transmission Electron Microscopy Grids to Direct Cell Positioning within Whole-Cell Cryo-Electron Tomography Workflows

Published on: September 13, 2021

7.7K
Microwave Assisted Rapid Diagnosis of Plant Virus Diseases by Transmission Electron Microscopy
09:20

Microwave Assisted Rapid Diagnosis of Plant Virus Diseases by Transmission Electron Microscopy

Published on: October 14, 2011

14.1K

Area of Science:

  • Materials Science
  • Image Processing
  • Electron Microscopy

Background:

  • Exit wavefunction reconstruction is crucial for high-resolution atomic imaging in transmission electron microscopy (TEM).
  • Existing iterative algorithms face limitations in accuracy and computational efficiency.
  • Commercial software like Trueimage offers solutions but can be computationally intensive.

Purpose of the Study:

  • To improve the performance of iterative wavefunction reconstruction algorithms.
  • To compare the enhanced algorithm against its conventional form and commercial software.
  • To accelerate computational speed using hardware programming.

Main Methods:

  • Implementing a wave propagation procedure for refining image alignment in the iterative algorithm.
  • Utilizing graphic processing unit (GPU) hardware programming with computer unified device architecture (CUDA).
  • Comparing reconstruction accuracy and efficiency with conventional methods and Trueimage software.

Main Results:

  • The enhanced iterative algorithm demonstrates improved accuracy in retrieving wavefunctions.
  • The algorithm retains advantages like requiring fewer images and allowing larger defocus steps.
  • GPU acceleration provides a 25-38 times speed increase compared to central processing unit (CPU) programming.

Conclusions:

  • The enhanced iterative wavefunction reconstruction algorithm offers superior performance for atomic-resolution TEM.
  • Wave propagation-based image alignment refinement is key to improved accuracy.
  • GPU acceleration makes complex reconstructions computationally feasible and efficient.