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HPLC-ESI-MS method for C60 fullerene mitochondrial content quantification.

Anna Grebinyk1,2,3, Sergii Grebinyk1, Svitlana Prylutska4

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This study quantifies C60 fullerene accumulation in human leukemic cell mitochondria. The developed HPLC-ESI-MS method enables precise measurement of this nanoparticle within cellular structures.

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Area of Science:

  • Nanotechnology
  • Cell Biology
  • Analytical Chemistry

Background:

  • Carbon nanoparticles, specifically C60 fullerene, are investigated for biomedical applications.
  • Understanding nanoparticle uptake and distribution in cells is crucial for assessing their biological impact.
  • Mitochondrial accumulation of nanoparticles can influence cellular function and viability.

Purpose of the Study:

  • To quantify the accumulation of C60 fullerene within the mitochondria of human leukemic cells.
  • To establish and validate a robust analytical method for C60 fullerene quantification in biological samples.
  • To provide data supporting research on fullerene interactions with cancer cells.

Main Methods:

  • Development of a high-performance liquid chromatography-electro spray ionization-mass spectrometry (HPLC-ESI-MS) method for C60 fullerene detection.
  • Incubation of human leukemic cells with C60 fullerene.
  • Cellular homogenization and differential centrifugation to isolate mitochondrial fractions.
  • Quantification of C60 fullerene in isolated mitochondrial extracts using HPLC-ESI-MS.

Main Results:

  • A validated HPLC-ESI-MS method was successfully established for precise C60 fullerene quantification.
  • The study successfully quantified C60 fullerene content within the mitochondrial fractions of treated human leukemic cells.
  • The data provides quantitative evidence of C60 fullerene accumulation in cellular mitochondria.

Conclusions:

  • The developed HPLC-ESI-MS method is suitable for quantifying C60 fullerene in cellular mitochondria.
  • This data contributes to understanding the intracellular fate of C60 fullerene in human leukemic cells.
  • The findings are relevant for further research into fullerene-based nanomedicine and photodynamic therapy.