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Microfluidic thread-based electrode system to detect glucose and acetylthiocholine.

Michelle Gaines1, Maria Jose Gonzalez-Guerrero1, Kathryn Uchida1

  • 1Department of Chemistry and Biochemistry, California State University, Los Angeles, CA, USA.

Electrophoresis
|September 21, 2018
PubMed
Summary
This summary is machine-generated.

This study presents a simple, reusable electrochemical sensor made from nylon thread for detecting glucose and acetylthiocholine. The sensor shows a linear response to varying concentrations of both analytes, demonstrating its potential for accurate biochemical analysis.

Keywords:
AcetylcholinesteraseDiagnostic deviceElectrochemical sensorGlucosePoint-of-care

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Area of Science:

  • Electrochemistry
  • Biosensors
  • Materials Science

Background:

  • Electrochemical sensors offer sensitive detection methods.
  • Developing reusable and easily fabricated sensors is crucial for practical applications.
  • Nylon thread presents a novel, low-cost substrate for electrochemical devices.

Purpose of the Study:

  • To develop and characterize a reusable electrochemical sensor using nylon thread.
  • To demonstrate the sensor's capability for detecting glucose and acetylthiocholine.
  • To evaluate the sensor's performance in terms of linearity and reproducibility.

Main Methods:

  • Fabrication of a three-electrode system (reference, working, counter) on nylon thread using conductive inks.
  • Immobilization of glucose oxidase (GOx) and potassium ferricyanide for glucose detection.
  • Immobilization of acetylcholinesterase (AChE) for acetylthiocholine detection.
  • Electrochemical measurements using cyclic voltammetry (CV).

Main Results:

  • The sensor exhibited a linear current output proportional to glucose concentration (R² = 0.985).
  • The sensor showed a linear current output proportional to acetylthiocholine concentration (R² = 0.995).
  • The developed sensor is reusable and simple to fabricate.

Conclusions:

  • A novel, cost-effective, and reusable electrochemical sensor based on nylon thread has been successfully developed.
  • The sensor demonstrates high sensitivity and linearity for both glucose and acetylthiocholine detection.
  • This thread-based sensor holds promise for various biochemical sensing applications.