Thread- and Capillary Tube-Based Electrodes for the Detection of Glucose and Acetylthiocholine

An electrochemical sensor for the detection of glucose and acetylthiocholine (ATC) using thread- and capillary tube-based electrodes is described. Three nylon thread-based electrodes were fabricated by painting pieces of trifurcated nylon thread with conductive inks and threading the electrodes into capillary tubes. Two platforms, one paper-based and the other utilizing bubble wrap, were examined. For the glucose detection, a solution containing glucose oxidase (GOx), potassium ferricyanide (K₃[Fe(CN)₆]), and increasing concentrations of glucose (0-20 mM) in phosphate-buffered saline (PBS) was spotted onto the two platforms. Similarly, increasing concentrations of ATC (0-9.84 mg/mL) in acetylcholinesterase (AChE) (0.08 U/mL) and PBS solution were detected. Using cyclic voltammetry (CV), a scanning voltage was applied to yield a graph of voltage applied (V) vs. current output (A). For both platforms, both glucose and ATC concentrations were observed to be linearly proportional to the current output as demonstrated by the increased height of the oxidation peaks. The three-electrode system was simple to fabricate, inexpensive, and could be used for multiple readings.