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Microfluidic Paper-based Analytical Devices (μPADs): Miniaturization and Enzyme Storage Studies.

Grenalynn Ilacas1, Frank A Gomez1

  • 1Department of Chemistry and Biochemistry, California State University.

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
|December 12, 2018
PubMed
Summary
This summary is machine-generated.

Miniaturized microfluidic paper-based analytical devices (μPADs) offer a stable platform for glucose assays and enzyme storage. These low-cost diagnostic tools show promise for point-of-care applications in resource-limited settings.

Keywords:
Miniaturizationdiagnostic deviceenzyme storageglucosepoint-of-care

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Area of Science:

  • Analytical Chemistry
  • Biotechnology
  • Materials Science

Background:

  • Microfluidic paper-based analytical devices (μPADs) are emerging as low-cost diagnostic tools.
  • Enzyme stability and assay performance in miniaturized formats are critical for practical applications.

Purpose of the Study:

  • To design and develop miniaturized μPADs for biological assays and enzyme storage.
  • To evaluate the performance and stability of these devices using glucose and acetylcholinesterase assays.

Main Methods:

  • Fabrication of two types of μPADs ('strip' and 'grid' designs) using wax printing on chromatographic paper.
  • Assessing glucose concentration using glucose oxidase (GOx), horseradish peroxidase (HRP), and potassium iodide (KI).
  • Evaluating acetylcholinesterase (AChE) activity and enzyme stability under various storage temperatures (-20, 0, 20°C) over 30 days.

Main Results:

  • Miniaturized μPADs demonstrated effective glucose detection with good correlation between color intensity and glucose concentration (0.89 - 15.76% error after 7 days).
  • The devices showed minimal enzyme denaturation after 30 days of storage at different temperatures.
  • Both 'strip' and 'grid' μPAD designs proved effective for biological assays.

Conclusions:

  • Miniaturized μPADs are promising for point-of-care diagnostics and enzyme storage.
  • These devices offer a viable alternative for resource-limited regions lacking advanced materials and instrumentation.
  • The developed μPADs exhibit stability and reliability for biological assays.