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Related Concept Videos

Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

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For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
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DNA Topoisomerases02:02

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Topoisomerases are enzymes that relax overwound DNA molecules during various cell processes, including DNA replication and transcription. These enzymes regulate positive and negative DNA supercoiling without changing the nucleotide sequence. DNA overwinding in a clockwise direction results in positively supercoiled DNA, whereas underwinding in a counterclockwise direction produces negatively supercoiled DNA.
Types and Mechanism of action
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DNA Helicases00:55

DNA Helicases

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DNA unwinding helicase enzymes are a type of motor protein. Motor proteins can translocate along filaments or polymers using energy generated from ATP hydrolysis. Helicases are involved in all the important cellular processes where DNA unwinding is required, such as DNA replication, repair, recombination, and transcription. They are present in all living organisms, but vary in their structure, function, and mechanism of action. For example, in prokaryotes, DnaB helicase binds and translocates...
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DNA-only Transposons02:57

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DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
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The DNA Replication Fork01:02

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An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
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3' End Sequencing Library Preparation with A-seq2
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Robust single-cell DNA methylome profiling with snmC-seq2.

Chongyuan Luo1,2, Angeline Rivkin1, Jingtian Zhou1

  • 1Genomic Analysis Laboratory, The Salk Institute for Biological Studies, La Jolla, CA, 92037, USA.

Nature Communications
|September 22, 2018
PubMed
Summary
This summary is machine-generated.

We developed snmC-seq2, a new method for single-cell DNA methylome profiling. This technique improves sequencing library quality, enabling more robust epigenomic studies in complex biological systems.

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Area of Science:

  • Epigenetics
  • Genomics
  • Molecular Biology

Background:

  • Single-cell DNA methylome profiling is crucial for understanding epigenomic heterogeneity.
  • Current methods face limitations due to modest sequencing library quality, hindering broader applications.

Purpose of the Study:

  • To introduce snmC-seq2, an improved method for single-cell DNA methylome profiling.
  • To enhance the quality and efficiency of single-cell epigenomic studies.

Main Methods:

  • Development and validation of the snmC-seq2 protocol.
  • Comparative analysis with the previous snmC-seq method.

Main Results:

  • snmC-seq2 demonstrates improved read mapping and reduced artifactual reads.
  • The new method offers enhanced throughput, library complexity, and coverage uniformity.
  • snmC-seq2 outperforms snmC-seq in key sequencing library quality metrics.

Conclusions:

  • snmC-seq2 is an efficient and high-quality strategy for large-scale single-cell epigenomic studies.
  • This advancement facilitates deeper investigation of epigenomic heterogeneity in various biological contexts.