A naphthalene-based fluorescent probe with a large Stokes shift for mitochondrial pH imaging
1College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China. smshuang@sxu.edu.cn.
The Analyst
|September 22, 2018
Summary
A novel fluorescent probe was developed to track pH changes within mitochondria. This probe effectively targets mitochondria and visualizes pH fluctuations in live cells, aiding cellular research.
Area of Science:
- Chemical Biology
- Mitochondrial Physiology
- Fluorescent Probes
Background:
- Mitochondria play a crucial role in cellular energy production and homeostasis.
- Accurate monitoring of mitochondrial pH is essential for understanding cellular function and disease.
- Existing probes may have limitations in targeting, sensitivity, or spectral properties.
Purpose of the Study:
- To synthesize and characterize a novel mitochondrial-targeted pH fluorescent probe.
- To evaluate the probe's performance in detecting pH changes within the physiological range of mitochondria.
- To demonstrate the probe's utility in visualizing mitochondrial pH dynamics in live cells.
Main Methods:
- Facile synthesis of the probe 4-(2-(6-hydroxynaphthalen-2-yl)vinyl)-1-methylpyridin-1-ium via carbon-carbon double bond bridging.
- Characterization of pH-dependent fluorescent behavior and determination of pKa value.
- Assessment of Stokes shift for reduced optical interference.
- Application in live HepG2 cells for mitochondrial targeting and pH visualization.
Main Results:
- Successful synthesis of a mitochondrial-targeted pH fluorescent probe.
- Probe exhibited remarkable pH-dependent fluorescence in the linear range of 7.60-10.00.
- A pKa value of 8.85 ± 0.04 was determined, suitable for mitochondrial pH.
- A large Stokes shift of 196 nm was achieved, minimizing excitation light interference.
- Excellent mitochondrial targeting and successful visualization of mitochondrial pH fluctuations in live HepG2 cells.
Conclusions:
- The synthesized probe is a valuable tool for monitoring mitochondrial pH.
- The probe's properties facilitate accurate and sensitive detection of pH changes in live cells.
- This probe has significant potential for applications in cell biology and biomedical research.
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