Related Experiment Video
Updated: Feb 5, 2026

Direct and Indirect Culture Methods for Studying Biodegradable Implant Materials In Vitro
Published on: April 15, 2022
UPLC-MS/MS method validation of ciprofloxacin in human urine: Application to biodegradability study in microbial fuel
Priya Sharma1, Devendra Kumar2, Srikanth Mutnuri1
1BITS Pilani, KK Birla Goa Campus, Applied Environmental Biotechnology Laboratory, Zuarinagar, Goa, India.
Abstract:
To enable the reliable quantification of ciprofloxacin in human urine, a sensitive and selective assay based on liquid chromatography-tandem mass spectrometry was developed. The chromatographic separation of the ciprofloxacin was carried out on a Zorbex Eclipse C18 column using methanol and ammonium acetate as a mobile phase by the gradient elution method. The developed assay covered a wide range of concentrations (1.56-100 ng/mL) with a lower limit of detection of 0.76 ng/mL. Quantification was performed using the multiple reaction monitoring transitions 331.8/231 for ciprofloxacin and 362/318 for ofloxacin (internal standard). This assay was validated for linearity, accuracy, precision and recovery. The validated method was then applied to the biodegradability of ciprofloxacin (99%) from human urine in the microbial fuel cell.
More Related Videos
16:33Methods for Facilitating Microbial Growth on Pulp Mill Waste Streams and Characterization of the Biodegradation Potential of Cultured Microbes
Published on: December 12, 2013
12:30Development and Validation of Chromium Getters for Solid Oxide Fuel Cell Power Systems
Published on: May 26, 2019
Related Concept Videos
Batteries and Fuel Cells
Urine Studies II: Urine Culture and Sensitivity Test
Urine Studies I: Urinalysis
Filtration and Urine Formation
Methods for Controlling Microbial Growth
Biological Methods for Microbial Control