Related Experiment Video
Updated: Feb 5, 2026
11:42
Hemogenic Reprogramming of Human Fibroblasts by Enforced Expression of Transcription Factors
Published on: November 4, 2019
6.5K
Genome Architecture Mediates Transcriptional Control of Human Myogenic Reprogramming.
Sijia Liu1, Haiming Chen2, Scott Ronquist2
1Department of Computational Medicine & Bioinformatics, University of Michigan, Ann Arbor, MI 48109, USA; Department of Electrical Engineering and Computer Science, University of Michigan, Ann Arbor, MI 48109, USA.
Iscience
|September 22, 2018
Summary
Genome architecture reorganization precedes cell identity changes during transcription factor (TF)-mediated reprogramming. These topological features are key to initiating transcriptional programs and cell fate transitions.
Area of Science:
- Genomics and Epigenetics
- Cellular Reprogramming and Differentiation
- Systems Biology
Background:
- Genome architecture plays a role in transcriptional regulation, but its influence on cell identity is unclear.
- Transcription factor (TF)-mediated reprogramming is a powerful tool for studying cell fate transitions.
Purpose of the Study:
- To investigate the interplay between genome architecture and transcriptional programs during myogenic reprogramming.
- To understand how topological features of genome architecture influence the initiation of cell identity.
Main Methods:
- Developed novel methods to evaluate topological features of genome architecture using network centrality.
- Integrated analysis of genome architecture and transcriptome dynamics during TF-mediated myogenic reprogramming of human fibroblasts.
Main Results:
- Significant reorganization of genome architecture topologically precedes the activation of myogenic transcriptional programs.
- This architectural reorganization occurs at multiple genomic scales, facilitating the definitive adoption of the myogenic phenotype.
- Transcription factors involved in the myogenic program subsequently entrain biological rhythms.
Conclusions:
- Topological features of genome architecture are crucial for initiating transcriptional programs during TF-mediated cellular reprogramming.
- This study highlights a novel role for genome architecture in controlling cell identity transitions.
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