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Updated: Feb 5, 2026

Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris
Published on: February 25, 2010
Continuous and Integrated Expression and Purification of Recombinant Antibodies
Sebastian Vogg1, Moritz Kai Florian Wolf1, Massimo Morbidelli2
1ETH Zurich, Institute for Chemical and Bioengineering, Zurich, Switzerland.
Abstract:
This chapter introduces the necessary concepts to design continuous expression and purification processes for monoclonal antibodies. The operation of a perfusion bioreactor is discussed containing the preparation procedures, the seeding train and the preparation and control of a long-term production run. The downstream processes exploit the benefits of countercurrent chromatography. Their design from batch experiments is presented. The CaptureSMB process is introduced for continuous capturing while for polishing applications the design of the two-column MCSGP process is described. The chapter also puts these processes together in the context of their integration to an end-to-end production process.
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