Related Experiment Video
Updated: Feb 4, 2026

Author Spotlight: Improved Nucleofection for High-Efficiency Gene Delivery in Murine Subventricular Zone-Derived Neural Stem Cell Cultures
Published on: June 14, 2024
Isolation of Extracellular Vesicles from Subventricular Zone Neural Stem Cells
Mary C Morton1, Victoria N Neckles1, David M Feliciano2
1Department of Biological Sciences, Clemson University, Clemson, SC, USA.
Abstract:
The neonatal subventricular zone (SVZ) is a neurogenic niche that contains neural stem cells (NSCs). NSCs release particles called extracellular vesicles (EVs) that contain biological material. EVs are transferred to cells, including immune cells in the brain called microglia. A standard approach to identify EV functions is to isolate and transplant EVs. Here, a detailed protocol is provided that will allow one to culture neonatal SVZ NSCs and to isolate, label, and transplant EVs. The protocol will permit careful and thorough examination of EVs in a wide range of physiological and pathophysiological conditions.
Related Concept Videos
Hybrid Zones
Zones of Protection
Protective zones are defined by closed dashed lines, containing one or more components. A key characteristic of these zones is the strategic placement of...
Adult Stem Cells
Embryonic Stem Cells
Transition Zone
Induced Pluripotent Stem Cells

