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The Functional Assessment of T cells
1Department of Immunology, Ehime University Graduate School of Medicine, Toon, Ehime, Japan. saho@m.ehime-u.ac.jp.
Abstract:
It is important to know what kind of T cell populations is involved in various disease states and to know the state of T cell functions involved in the disease. When T cell antigen receptors (TCR) recognize a specific antigen, the cell transmits a signal by a transduction mechanism within the T cell's cytoplasm. This signal initiates gene transcription essential for differentiation and activation of T cells. In this chapter, we will describe the methods of analyzing the transcribed mRNA and detecting the translated product in order to know the activation state of T cells.
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