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LC3-Associated Phagocytosis and Antigen Presentation.
Laure-Anne Ligeon1, Monica Loi1, Christian Münz1
1Department of Viral Immunobiology, Institute of Experimental Immunology, University of Zurich, Zurich, Switzerland.
Current Protocols in Immunology
|September 26, 2018
Summary
LC3-associated phagocytosis (LAP) is an unconventional autophagy pathway. This study details methods to quantify LAP formation and validate its role in human macrophage immune responses.
Area of Science:
- Immunology
- Cell Biology
- Autophagy Research
Background:
- LC3-associated phagocytosis (LAP) is a unique autophagy process utilizing canonical autophagy machinery.
- LAP is initiated by receptor-mediated phagocytosis, forming LC3-decorated single-membrane vesicles.
- The molecular mechanisms of LAP in professional phagocytes like macrophages are not fully understood.
Purpose of the Study:
- To describe common methods for monitoring and quantifying LC3-coated endosomes (LAPosomes).
- To validate the involvement of LAP in immunological processes within human macrophages.
Main Methods:
- Utilizing established techniques to observe and measure LAPosome formation.
- Applying these methods to assess LAP's role in human macrophage immunology.
Main Results:
- The study presents validated methodologies for LAP research.
- Demonstrates the utility of these methods in studying LAP in human macrophages.
Conclusions:
- The described methods provide a framework for further investigation into LAP.
- These techniques aid in understanding LAP's contribution to immune functions in macrophages.
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