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[Conjugative R-plasmid of facultative methylotrophic bacteria Pseudomonas sp. M]
Abstract:
50 Md conjugative plasmid, designated pM3, has been found in the cells from natural isolates of Pseudomonas sp M. The plasmid determines the resistance to tetracycline and streptomycin and is capable of conjugative transfer between the cells of Pseudomonas and Escherichia coli. The conjugative derivatives of pM3 deleted for 14 Md of molecular mass were isolated after acridine dyes treatment of cells harbouring plasmid pM3. The discovered plasmid was not shown to belong to IncP1 incompatibility group.
Insights
A 50 megadalton conjugative plasmid, pM3, was identified in Pseudomonas sp M, conferring resistance to tetracycline and streptomycin. This plasmid can transfer between Pseudomonas and Escherichia coli, with derivatives showing reduced molecular mass.
Area of Science:
- Molecular Biology
- Microbiology
- Genetics
Context:
- Natural isolates of Pseudomonas sp M harbor a novel 50 megadalton (Md) conjugative plasmid, designated pM3.
- This plasmid confers resistance to critical antibiotics, tetracycline and streptomycin.
- pM3 exhibits conjugative transfer capabilities, facilitating gene exchange between Pseudomonas and Escherichia coli.
Purpose:
- To characterize the newly discovered 50 Md conjugative plasmid, pM3, found in Pseudomonas sp M.
- To investigate the antibiotic resistance profile and transferability of the pM3 plasmid.
- To determine the incompatibility group of the pM3 plasmid.
Summary:
- The 50 Md conjugative plasmid pM3 was isolated from natural Pseudomonas sp M.
- pM3 confers resistance to tetracycline and streptomycin and is transferable between Pseudomonas and E. coli.
- Treatment with acridine dyes yielded pM3 derivatives with reduced molecular mass (14 Md deletion); the plasmid does not belong to the IncP1 incompatibility group.
Impact:
- Characterization of pM3 provides insights into plasmid-mediated antibiotic resistance mechanisms in Pseudomonas.
- Understanding the conjugative transfer of pM3 contributes to knowledge of horizontal gene transfer dynamics.
- The findings expand the understanding of plasmid diversity beyond known incompatibility groups.