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Updated: Feb 4, 2026

Denaturing Gradient Gel Electrophoresis DGGE
Published on: February 25, 2007
Applications of Difference Gel Electrophoresis (DIGE) in the Study of Microorganisms
Kathleen Trautwein1, Ralf Rabus2
1Institute for Chemistry and Biology of the Marine Environment (ICBM), Carl von Ossietzky University Oldenburg, Oldenburg, Germany.
Abstract:
OMICs-based investigations of microorganisms are becoming more and more widespread in the upcoming era of systems and synthetic biology. Here, proteomics plays a key role and two-dimensional difference gel electrophoresis (2D DIGE) remains the "gold-standard" for globally determining protein abundance changes on a quantitative and statistically confident level-in particular also for laboratories not having full-cycle proteomic facilities at their disposal. In this contribution we summarize our methodological procedures and experiences with 2D DIGE accumulated over the past 15 years.
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