Peroxidase release from rat submandibular salivary acinar cells in vitro

Acinar cell aggregates were isolated and then incubated for 60 min with or without isoproterenol, phenylephrine, carbamylcholine, and insulin, and peroxidase activity in the cells and medium assayed. Isoproterenol stimulated the release of 70-80 per cent of total peroxidase activity. Carbamylcholine stimulation resulted in the release of only 15 per cent of this activity; phenylephrine had no effect. Insulin alone failed to stimulate peroxidase secretion, but it potentiated the response of the cells to carbamylcholine.