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Analytical Techniques for Assaying Nitric Oxide Bioactivity
Published on: June 18, 2012
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Non-touching plasma-liquid interaction - where is aqueous nitric oxide generated?
Helena Jablonowski1, Ansgar Schmidt-Bleker, Klaus-Dieter Weltmann
1ZIK Plasmatis at Leibniz Institute for Plasma Science and Technology (INP Greifswald e.V.), Felix-Hausdorff-Str. 2, 17489 Greifswald, Germany. helena.jablonowski@inp-greifswald.de.
Physical Chemistry Chemical Physics : PCCP
|September 29, 2018
Summary
This study investigated nitric oxide (NO) delivery from plasma jets into liquids. Researchers quantified NO and its precursors, identifying pathways to enhance NO production for potential therapeutic applications.
Area of Science:
- Plasma medicine
- Biomedical engineering
- Free radical chemistry
Background:
- Nitric oxide (NO) is a crucial signaling molecule in biological systems, involved in inflammation, wound healing, and cancer.
- Non-thermal plasma jets generate NO, offering potential for clinical applications, but its efficient transfer into liquids and tissues is unclear.
Purpose of the Study:
- To determine nitric oxide concentration in buffered solutions after plasma treatment.
- To identify the origin and formation pathways of NO in liquid.
- To explore methods for enhancing NO production by altering plasma gas composition.
Main Methods:
- Electron paramagnetic resonance (EPR) spectroscopy was used to quantify nitric oxide concentration in buffered solutions.
- Detection of potential NO precursors including hydroxyl radicals, superoxide anions, atomic hydrogen, nitrite, nitrate, and hydrogen peroxide.
- Analysis of gas composition (feed and surrounding) during plasma treatment of liquids.
Main Results:
- The direct origin of NO in the liquid phase was confirmed, ruling out indirect formation from detected precursors.
- Various reactive oxygen and nitrogen species (RONS) and stable products were identified.
- Potential pathways for NO formation and enhancement strategies were elucidated through gas composition adjustments.
Conclusions:
- Electron paramagnetic resonance spectroscopy is effective for quantifying NO in plasma-treated liquids.
- Understanding NO transport and formation mechanisms is key to optimizing plasma-based therapies.
- Alteration of gas composition presents a viable strategy for enhancing NO production in plasma-liquid interactions.
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