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A Label-Free Fluorescent AND Logic Gate Aptasensor for Sensitive ATP Detection.
Jingjing Zhang1, Chunzheng Yang2, Chaoqun Niu3
1State Key Laboratory of Marine Resource Utilization in South China Sea, College of Information Science & Technology, College of Materials and Chemical Engineering, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China. zhangjingjingaoxue@163.com.
Sensors (Basel, Switzerland)
|October 3, 2018
Summary
A novel aptasensor was developed for sensitive detection of adenosine triphosphate (ATP). This simple, label-free biosensor successfully constructed an AND logic gate for accurate ATP quantification.
Area of Science:
- Biosensor technology
- Nucleic acid aptamers
- Graphene oxide applications
Background:
- Adenosine triphosphate (ATP) is a crucial molecule in cellular energy transfer and signaling.
- Accurate detection of ATP is vital for understanding various biological processes.
- Existing ATP detection methods may lack sensitivity, simplicity, or cost-effectiveness.
Purpose of the Study:
- To develop a label-free, enzyme-free aptasensor for adenosine triphosphate (ATP) detection.
- To construct an AND logic gate biosensor for enhanced detection specificity.
- To evaluate the performance of the developed aptasensor in terms of sensitivity, linearity, and specificity.
Main Methods:
- Fabrication of an aptasensor probe by immobilizing double-stranded DNA on graphene oxide (GO).
- Utilizing ATP and single-stranded DNA as input signals.
- Measuring fluorescence intensity changes of PicoGreen dye as the output signal.
Main Results:
- Successful construction of an AND logic gate aptasensor.
- Linear detection range for ATP from 20 to 400 nM (R² = 0.9943).
- Achieved a limit of detection (LOD) of 142.6 pM and high sensitivity.
Conclusions:
- The developed aptasensor offers a simple, cost-effective, and highly sensitive method for ATP detection.
- The AND logic gate functionality enhances the reliability of ATP detection.
- This biosensor holds potential for various biological and diagnostic applications requiring ATP monitoring.
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