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Anfractuous assemblies of IMP dehydrogenase and CTP synthase: new twists on regulation?
Gregory D McCluskey1, Stephen L Bearne1,2
1Department of Biochemistry & Molecular Biology, Dalhousie University, Halifax, Canada.
Abstract:
CTP synthase (CTPS) and IMP dehydrogenase (IMPDH) catalyse the rate-limiting steps of de novo CTP and guanosine nucleotide biosynthesis, respectively, and form filament assemblies in response to inhibitors. A recent study explores the morphology and dynamics of these assemblies using fluorescence and super-resolution confocal microscopy with cell lines expressing CTPS1 and IMPDH2 fusion proteins. The formation and dismantling of mixed assemblies depends on nucleotide levels, suggesting a co-regulation function.
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