Related Experiment Video
Updated: Feb 4, 2026

Quantitative Methods to Study Protein Arginine Methyltransferase 1-9 Activity in Cells
Published on: August 7, 2021
Photoluminescent arginine-functionalized polycitrate with enhanced cell activity and hemocompatibility for live cell
Min Wang1, Yi Guo1, Peter X Ma2,3
1Frontier Institute of Science and Technology, State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an, 710054, China.
Abstract:
Development of biodegradable and highly biocompatible polymer with intrinsical photoluminescence and high photostability for real-time live cell bioimaging has attracted much attention recently. Here, a biodegradable and amphiphilic poly (citrate)-co-poly (ethylene glycol) (PEG) grafted with arginine (PCGA) polymer with intrinsical fluorescence was synthesized for targeted live cell bioimaging. The physicochemical structure, photoluminescent properties, hemocompatibility, cytotoxicity, and fluorescent bioimaging studies in live cells were determined in detail. PCGA showed a significantly high hemocompatibility, low cytotoxicity, and excellent photostability, which allows for imaging the live cells attachment and proliferation. Furthermore, PCGA could efficiently enhance cell attachment and proliferation due to the presence of arginine, suggesting their high cellular biocompatibility. Importantly, PCGA could selectively stain the lysosome in cells. Our results demonstrated that the amino acid-based polymer functionalization may be an important strategy to develop multifunctional biomaterials with enhanced biocompatibility for targeted bioimaging, cancer therapy, and regenerative medicine. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 3175-3184, 2018.
Related Concept Videos
Photoluminescence: Applications
Photoluminescence: Fluorescence and Phosphorescence
A pair of electrons in a...
B Cell Activation and Differentiation
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
Protein Dynamics in Living Cells
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
T Cell Types and Functions
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
T Cell Activation and Clonal Selection
Naive T cells that have not yet encountered an antigen express two primary CD...

