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Related Experiment Video

Updated: Feb 4, 2026

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Published on: February 9, 2012

A four-state fluorescent molecular switch.

Yuan Liu¹, Jiawang Zhou, Michael R Wasielewski

¹Department of Chemistry and International Institute for Nanotechnology, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208, USA. chadnano@northwestern.edu hangxing@hnu.edu.cn.

Chemical Communications (Cambridge, England)

|October 9, 2018

Summary

This summary is machine-generated.

This study demonstrates a single molecular construct capable of four distinct fluorescent states. Fluorescence is controlled by reversible coordination chemistry at a platinum(II) center in solution and solid phases.

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Area of Science:

Supramolecular Chemistry
Materials Science
Photophysics

Background:

Fluorescent materials offer versatile applications in sensing and imaging.
Controlling multiple fluorescence states in a single molecule remains a significant challenge.
Coordination chemistry provides a pathway for tuning molecular properties.

Purpose of the Study:

To develop a single molecular construct exhibiting multiple, distinct fluorescent states.
To investigate the manipulation of fluorescence through reversible coordination chemistry.
To explore the behavior of the construct in both solution and solid phases.

Main Methods:

Synthesis of a Weak-Link Approach (WLA) construct incorporating pyrene and tetraphenylethene moieties.
Characterization of the construct's photophysical properties.
Investigation of fluorescence modulation via reversible coordination at a platinum(II) center.

Main Results:

Achieved four distinct fluorescent states within the single WLA construct.
Demonstrated successful manipulation of fluorescence in both solution and solid states.
Confirmed the role of reversible coordination chemistry at the Pt(II) center in controlling fluorescence.

Conclusions:

The developed WLA construct offers a novel platform for multi-state fluorescence.
Reversible coordination chemistry is an effective strategy for tuning the photophysical properties of such systems.
This work opens avenues for advanced fluorescent materials with tunable emission properties.