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Microbial forensics: new breakthroughs and future prospects.

Manuela Oliveira1,2,3, António Amorim4,5,6

  • 1i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, 4200-135, Porto, Portugal. manuelao@ipatimup.pt.

Applied Microbiology and Biotechnology
|October 11, 2018
PubMed
Summary
This summary is machine-generated.

Massive parallel sequencing (MPS) advances microbial forensics, enabling new applications from criminal cases to human identification. Further development of standards and databases is crucial for its forensic implementation.

Keywords:
Criminal investigationGeneticsMassive parallel sequencingMicrobial forensicsMicrobiome

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Area of Science:

  • Microbial Forensics
  • Forensic Microbiology
  • Genomic Analysis

Background:

  • Advances in genetic data generation, storage, and analysis via massive parallel sequencing (MPS) have significantly progressed microbial forensics.
  • Traditional applications in biocrime, bioterrorism, and epidemiology are expanding.

Purpose of the Study:

  • To explore the expanding applications of microbial forensics.
  • To highlight the advantages of MPS over classical microbiological methods.
  • To identify necessary steps for forensic implementation.

Main Methods:

  • Massive Parallel Sequencing (MPS) for microbial genetic data generation.
  • Comparative analysis of MPS with classical microbiological techniques.
  • Review of current and potential applications in forensic science.

Main Results:

  • MPS offers diverse advantages and alternative possibilities compared to classical methods.
  • Potential applications include ancillary evidence, cause of death clarification, human identification, geolocation, and postmortem interval estimation.
  • Significant progress has been made in microbial forensics.

Conclusions:

  • Microbial forensics, powered by MPS, has a broad range of potential applications.
  • Development of standards, guidelines, and comprehensive reference databases is essential for widespread forensic adoption.