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Combination of Fluoride and SO2 Induce DNA Damage and Morphological Alterations in Male Rat Kidney

Yan Gao1, Chen Liang1, Jianhai Zhang1

  • 1Shanxi Key Laboratory of Ecological Animal Science and Environmental Veterinary Medicine, College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, China.

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
|October 12, 2018
PubMed
Abstract

Insights

Combined exposure to sodium fluoride (NaF) and sulfur dioxide (SO2) induces kidney damage and DNA alterations in rats. This synergistic effect shows a time and dose correlation, highlighting potential health risks.

Area of Science:

  • Toxicology
  • Environmental Health
  • Molecular Biology

Background:

  • Investigating the combined toxic effects of sodium fluoride (NaF) and sulfur dioxide (SO2).
  • Assessing kidney morphological changes and DNA damage in male Wistar rats.

Purpose of the Study:

  • To determine the synergistic toxic effect of NaF and SO2 on rat kidneys.
  • To evaluate kidney morphology and DNA integrity following co-exposure.

Main Methods:

  • 96 male Wistar rats were divided into four groups: control, NaF, SO2, and NaF + SO2.
  • Kidney morphology assessed via H&E staining at 2, 4, 6, and 8 weeks.
  • DNA damage evaluated using Comet Assay (tailing ratio, comet length).

Main Results:

  • Treated rats showed decreased body weight; no significant kidney weight difference.
  • Morphological alterations in renal tubules observed in all treated groups, most pronounced with co-exposure.
  • Significant DNA damage detected in treated groups by 4 and 6 weeks.

Conclusions:

  • NaF and SO2 co-exposure induces renal tissue lesions and impacts DNA integrity.
  • The combined exposure exhibits a synergistic, dose-dependent, and time-correlated effect.
  • Findings suggest a potential mechanism involving DNA damage perturbations, necessitating cautious interpretation of co-exposure risks.
Keywords:
Comet assayDNA damageH&EKidneyRatSodium fluorideSulfur dioxide

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