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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
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Cis-regulatory Sequences02:02

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Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
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Sequences01:29

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Sequences are fundamental mathematical objects consisting of ordered lists of numbers that follow a specific rule or pattern. Sequences are critical in various mathematical concepts, including calculus, series, and number theory. They can model real-world phenomena such as population growth, financial investments, and physical processes like the diminishing height of a bouncing ball.Each number in a sequence is referred to as a term. Typically, the terms are denoted as a1, a2, a3,…, where...
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Researchers have tested many persuasion strategies, including the foot-in-the door and the door-in-the-face techniques, in a variety of contexts. Ultimately, the principles are effective in selling products and changing people’s attitude, ideas, and behaviors (Cialdini & Goldstein, 2004).
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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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An arithmetic sequence is a structured arrangement of numbers where each term is derived by adding a constant value, known as the common difference, to the previous term. This consistent pattern allows for the efficient computation of any term within the sequence as well as the cumulative sum of multiple terms. The formula for finding the nth term of an arithmetic sequence is:Here, aₙ represents the nth term of the sequence, a is the first term, d is the common difference, and n is the...
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Next-Generation Sequencing Strategies.

Shawn E Levy1, Braden E Boone1

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Next-generation sequencing (NGS) has rapidly advanced DNA and RNA sequencing, making genome-scale studies routine. This evolution drives progress in personalized medicine, evolution, and biodiversity research.

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Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • The advent of "next-generation" sequencing revolutionized RNA and DNA analysis.
  • Significant technological progress has exponentially increased sequencing capacity and reduced costs.
  • Genome-scale sequencing is becoming standard practice, shifting genomic research paradigms.

Purpose of the Study:

  • To review recent technological advancements in next-generation sequencing (NGS).
  • To highlight current large-scale sequencing initiatives.
  • To discuss the impact of NGS on genomics and related fields.

Main Methods:

  • Review of technological innovations in DNA and RNA sequencing.
  • Analysis of current large-scale genomic studies.
  • Synthesis of the impact of high-throughput sequencing on scientific discovery.

Main Results:

  • Rapid evolution of sequencing technologies with increased throughput and decreased cost.
  • Emergence of large-scale genomic studies as a standard approach.
  • Demonstration of NGS's impact on personalized medicine, evolution, and biodiversity.

Conclusions:

  • Next-generation sequencing has transformed genomics, enabling unprecedented large-scale studies.
  • Continued advancements in sequencing technology are crucial for future discoveries.
  • The integration of NGS is driving significant progress across diverse biological disciplines.