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Spray-Coated Melanin/PEDOT:PSS Films for Sustainable Organic Electrochemical Transistors
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PEDOT:PSS-based Multilayer Bacterial-Composite Films for Bioelectronics.

Tom J Zajdel1,2, Moshe Baruch2, Gábor Méhes2,3

  • 1Department of Electrical Engineering and Computer Sciences, University of California, Berkeley, Berkeley, California, United States of America.

Scientific Reports
|October 18, 2018
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel multilayer conductive bacterial-composite film (MCBF) to overcome biofilm thickness limitations. This advancement significantly boosts current output for microbial electrochemical systems, enabling better bioelectronics and biosensing applications.

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Area of Science:

  • Microbial electrochemistry
  • Bioelectronics
  • Biosensing

Background:

  • Microbial electrochemical systems (MES) offer eco-friendly energy conversion for wastewater treatment and bioelectronics.
  • Limited biofilm thickness hinders signal-to-noise ratios in biosensors and bioelectronics, requiring larger devices.
  • Developing thicker, functional biofilms is crucial for miniaturization and deployment of bioelectronic devices.

Purpose of the Study:

  • To create a thicker biofilm structure for enhanced signal generation in biosensors.
  • To embed electroactive bacteria within a conductive matrix for improved electron transfer.
  • To develop a versatile method for creating multilayer conductive bacterial-composite films (MCBFs).

Main Methods:

  • Embedding electroactive bacteria (Shewanella oneidensis MR-1) within a conductive poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) matrix.
  • Utilizing a flow-through method for bacterial encapsulation, achieving over 90% viability.
  • Electropolymerizing the composite film on a carbon felt substrate to form multilayer conductive bacterial-composite films (MCBFs) up to 80 µm thick.

Main Results:

  • MCBFs exhibited a tightly interleaved structure of bacteria and conductive PEDOT:PSS.
  • Shewanella oneidensis within MCBFs demonstrated efficient direct and riboflavin-mediated electron transfer.
  • MCBFs generated 20 times more steady-state current compared to native biofilms in bioelectrochemical reactors.

Conclusions:

  • The developed MCBF approach effectively increases biofilm thickness and current output.
  • This method allows for controlled fabrication of bacterial composite films for enhanced performance in MES.
  • The technology has direct applications in environmental sensing and miniaturized organic electronics.