Multi-stage countercurrent process for extracting protein from Antarctic Krill (Euphausia superba)

Area of Science:

• Biotechnology
• Food Science
• Marine Resources

Background:

• Antarctic krill (Euphausia superba) is a rich protein source.
• Efficient protein extraction is crucial for utilizing krill resources.
• Existing methods may not be optimal for large-scale protein recovery.

Purpose of the Study:

• To systematically study and optimize a multi-stage countercurrent process for Antarctic krill protein extraction.
• To enhance the efficiency and yield of krill protein recovery.

Main Methods:

• Investigated protein solubility in multi-step dissolution at pH 12.5.
• Evaluated multi-step cross-flow processing for extraction feasibility.
• Implemented and optimized a three-stage countercurrent extraction system.

Main Results:

• Protein solubility reached 33.0 ± 0.8 mg/mL at pH 12.5.
• The three-stage countercurrent method achieved 95.1 ± 0.6% protein extraction.
• A total recovery yield of 67.9 ± 1.6% was obtained after precipitation at pH 4.5.

Conclusions:

• Multi-stage countercurrent technology is feasible and effective for Antarctic krill protein extraction.
• The optimized process offers high protein recovery with efficient water usage.
• This technology provides a sustainable approach for maximizing krill protein utilization.