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Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics
Published on: October 13, 2020
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Recent advances in single-cell analysis by mass spectrometry.
Lei Yin1, Zhi Zhang, Yingze Liu
1Research Institute of Translational Medicine, The First Hospital of Jilin University, Jilin University, Dongminzhu Street, Changchun 130061, PR China. gujk@jlu.edu.cn.
The Analyst
|October 19, 2018
Summary
Mass spectrometry enables detailed single-cell analysis, overcoming challenges of small sample size and low substance concentrations. This review highlights advances in mass spectrometry techniques for cellular and subcellular investigations.
Area of Science:
- Biochemistry
- Cell Biology
- Analytical Chemistry
Background:
- Cells are fundamental units of life, requiring precise analysis for understanding metabolism and behavior.
- Single-cell analysis is vital for lipidomic and proteomic studies but faces challenges due to sample size and analyte concentration.
- Understanding cellular and subcellular mechanisms requires detailed structural and compositional knowledge.
Purpose of the Study:
- To review recent advancements in single-cell analysis using mass spectrometry.
- To classify and discuss various mass spectrometry ionization strategies for single-cell applications.
- To provide insights into the capabilities of mass spectrometry for elucidating molecular profiles at the cellular level.
Main Methods:
- Electrospray ionization mass spectrometry (ESI-MS)
- Secondary ion mass spectrometry (SIMS)
- Laser-based mass spectrometry
- Inductively coupled plasma mass spectrometry (ICP-MS)
Main Results:
- Mass spectrometry offers high sensitivity and selectivity for single-cell analysis.
- Diverse mass spectrometry techniques have been developed for cellular molecular profiling.
- Different ionization modes are being optimized for effective single-cell analysis.
Conclusions:
- Mass spectrometry is a powerful tool for overcoming single-cell analysis challenges.
- Continued development of mass spectrometry techniques enhances cellular and subcellular investigations.
- This review categorizes and analyzes strategies for mass spectrometry-based single-cell analysis.
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