Understanding the elusive protein corona of thermoresponsive nanogels
Enrico Miceli1,2, Benno Kuropka1, Christine Rosenauer3
1Freie Universität Berlin, Institute of Chemistry & Biochemistry, Takustr. 3, 14195 Berlin, Germany.
Aim:
We analyzed the protein corona of thermoresponsive, poly(N-isopropylacrylamide)- or poly(N-isopropylmethacrylamide)-based nanogels.
Materials & Methods:
Traces of protein corona detected after incubation in human serum were characterized by proteomics and dynamic light scattering in undiluted serum.
Results:
Apolipoprotein B-100 and albumin were the main components of the protein coronae. For dendritic polyglycerol-poly(N-isopropylacrylamide) nanogels at 37°C, an increase in adsorbed immunoglobulin light chains was detected, followed by partially reversible nanogel aggregation. All nanogels in their hydrophilic state are colloidally stable in serum and bear a dysopsonin-rich protein corona.
Conclusion:
We observed strong changes in NG stability upon slight alterations in the composition of the protein coronae according to nanogel solvation state. Nanogels in their hydrophilic state possess safe protein coronae.
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