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Screening Protein Aggregation in Cells Using Fluorescent Labels Coupled to Flow Cytometry.

Salvador Ventura1,2, Susanna Navarro3,4

  • 1Institut de Biotecnologia i de Biomedicina, Universitat Autònoma de Barcelona, Barcelona, Spain.

Methods in Molecular Biology (Clifton, N.J.)
|October 21, 2018
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Summary

This study introduces a rapid flow cytometry method to detect and quantify intracellular amyloid inclusions in simple cell models. This technique aids in studying protein misfolding and screening for anti-amyloid compounds in vivo.

Keywords:
AmyloidBacteriaFlow cytometryProtein aggregationProtein inclusionsYeast

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Neuroscience

Background:

  • Protein misfolding and aggregation into amyloid structures are implicated in neurodegenerative diseases.
  • Studying amyloid self-assembly within living cells requires robust methods beyond in vitro approaches.
  • Cellular models like bacteria and yeast offer convenient systems for investigating protein inclusions.

Purpose of the Study:

  • To develop a simple and fast flow cytometry method for detecting intracellular amyloid inclusions.
  • To analyze the distribution of amyloidogenic proteins within intact cells.
  • To enable quantification of intracellular amyloid content and screening of therapeutic compounds.

Main Methods:

  • Utilized flow cytometry for high-throughput analysis of intact cells.
  • Employed fluorescent amyloid-specific dyes (thioflavin-S, ProteoStat) and fluorescent protein fusions (GFP).
  • Applied the method to simple cellular models (bacteria, yeast) expressing recombinant amyloidogenic proteins.

Main Results:

  • Successfully detected and quantified intracellular amyloid inclusions using flow cytometry.
  • Demonstrated the method's ability to analyze protein distribution within cells.
  • Validated the use of specific fluorescent probes for amyloid detection.

Conclusions:

  • The developed flow cytometry method is effective for studying intracellular amyloid formation in vivo.
  • This technique facilitates the screening of anti-amyloidogenic compounds and the investigation of factors influencing amyloid deposition.
  • Simple cellular models combined with flow cytometry provide a powerful platform for amyloid research.