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Chondrogenic Differentiation Induction of Adipose-derived Stem Cells by Centrifugal Gravity
Published on: February 24, 2017
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Atomic Force Microscopy-Based Nanoscopy of Chondrogenically Differentiating Human Adipose-Derived Stem Cells:
Jie Yang1, Ming-Tang He2, Xun Huang1,3
1Institute of Orthopedic Diseases and Center for Joint Surgery and Sports Medicine, the First Affiliated Hospital, Jinan University, Guangzhou, 510630, People's Republic of China.
Nanoscale Research Letters
|October 25, 2018
Summary
Atomic force microscopy revealed increased binding probability between integrin β1 ligand and CD29 receptors on human adipose-derived stem cells during chondrogenesis. This study highlights AFM
Area of Science:
- Biophysics
- Stem Cell Biology
- Biomaterials Science
Background:
- Integrin β1 plays a crucial role in cell differentiation, migration, proliferation, and tissue development.
- Understanding integrin-ligand interactions is vital for stem cell differentiation and tissue engineering.
- Cluster of differentiation 29 (CD29) is the β1 integrin subunit receptor.
Purpose of the Study:
- To analyze the binding probability between integrin β1 ligand and CD29 receptors on human adipose-derived stem cells (hADSc) during chondrogenic differentiation.
- To investigate changes in hADSc morphology and membrane ultrastructure using atomic force microscopy (AFM) during chondrogenesis.
- To correlate integrin β1 binding with the β-catenin/SOX signaling pathway in chondrogenic differentiation.
Main Methods:
- Utilized atomic force microscopy (AFM) with integrin β1-functionalized tips to probe ligand-receptor interactions on hADSc.
- Measured cell morphology and ultrastructure of hADSc cultured in vitro during early chondrogenic differentiation.
- Recorded 1200 force curves at 0, 6, and 12 days of chondrogenic induction to quantify binding events and rupture forces.
Main Results:
- AFM demonstrated a transition in hADSc morphology from spindled to polygonal cells with altered length/width ratios and increased roughness during chondrogenic induction.
- The binding probability between integrin β1 ligand and CD29 receptors on hADSc significantly increased during chondrogenic induction (from 19.58% to 33.4%).
- Average rupture forces showed minor fluctuations (61.8 pN to 67.2 pN), while rupture event frequency increased, indicating enhanced ligand-receptor binding.
Conclusions:
- AFM provides kinetic and visual insights into integrin β1 ligand-CD29 receptor binding dynamics during chondrogenesis.
- Changes in cellular morphology, membrane ultrastructure, and ligand-receptor binding probability serve as valuable markers for evaluating chondrogenic differentiation.
- Integrin β1 interactions are correlated with the β-catenin/SOX signaling pathway, suggesting a role in chondrogenic differentiation regulation.
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