Aerobic biodegradation of HMX by Planomicrobium flavidum

In this report, aerobic biodegradation of octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine or high melting explosive (HMX), a highly explosive chemical by Planomicrobium flavidum strain S5-TSA-19, an isolate from an explosive-contaminated soil, was investigated. The isolate S5-TSA-19 degraded 70% of HMX in 20 days during which time nitrite ion was produced with the subsequent formation of metabolites, viz. methylenedintramine and N-methyl-N,N'-dinitromethanediamine with molecular weights 136 Da and 149 Da, respectively. The degradation mechanism was found to follow first-order kinetics with a half-life of 11.55 days and formation of above intermediates indicate single nitrite elimination pathway. The proliferation of isolate S5-TSA-19 in the absence of nitramines indicates the cometabolic degradation of HMX. Isolate S5-TSA-19 can thus be used as futuristic microbe for degradation of HMX at explosive-contaminated site.