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Updated: Feb 3, 2026
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Substrate Generation for Endonucleases of CRISPR/Cas Systems
Published on: September 8, 2012
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CRISPR-Based Technologies: Impact of RNA-Targeting Systems.
1Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, USA; Department of Genetics, University of Georgia, Athens, GA 30602, USA; Department of Microbiology, University of Georgia, Athens, GA 30602, USA.
Molecular Cell
|November 3, 2018
Summary
RNA-targeting CRISPR-Cas systems offer programmable tools for RNA manipulation. Recent advances enable novel biotechnological and biomedical applications by converting these systems into powerful RNA-binding and cleavage agents.
Area of Science:
- Molecular Biology
- Biotechnology
- Genetics
Background:
- DNA-targeting CRISPR-Cas systems (e.g., Cas9, Cas12) have transformed genome editing and gene expression control.
- RNA-targeting CRISPR-Cas systems represent a complementary approach to nucleic acid manipulation.
Purpose of the Study:
- To summarize current knowledge on RNA-targeting CRISPR-Cas systems.
- To highlight recent advancements in developing these systems as programmable RNA-binding and cleavage tools.
Main Methods:
- Review of existing literature on RNA-targeting CRISPR-Cas systems.
- Analysis of recent technological developments and applications.
Main Results:
- RNA-targeting CRISPR-Cas systems can be engineered for precise RNA binding and cleavage.
- These systems demonstrate versatility across various biotechnological and biomedical applications.
Conclusions:
- RNA-targeting CRISPR-Cas systems are emerging as powerful tools for RNA-based research and therapeutics.
- Further development promises significant impact in biotechnology and biomedicine.
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