Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Computed Tomography01:10

Computed Tomography

8.3K
Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...
8.3K
Positron Emission Tomography01:29

Positron Emission Tomography

7.2K
Positron emission tomography (PET) is a medical imaging technique involving radiopharmaceuticals — substances that emit short-lived radiation. Although the first PET scanner was introduced in 1961, it took 15 more years before radiopharmaceuticals were combined with the technique and revolutionized its potential.
One of the main requirements of a PET scan is a positron-emitting radioisotope, which is produced in a cyclotron and then attached to a substance used by the part of the body...
7.2K
Extraction: Effects of pH00:53

Extraction: Effects of pH

1.4K
Consider a neutral form of an amine, B, with a partition coefficient, K, in a liquid mixture containing organic and aqueous phases. The pH of the aqueous phase affects the charge on acidic and basic solutes, and the charged form is usually more soluble in the aqueous phase. Suppose the conjugate acid form of the amine is soluble only in the aqueous phase while the base form is soluble in both phases. Then the distribution coefficient, D, can be given as the ratio of amine concentration in the...
1.4K
Spinal Nerves: Plexus II01:21

Spinal Nerves: Plexus II

2.5K
The plexuses of the lower body include the lumbar, sacral, and coccygeal plexuses, which innervate the abdomen, pelvis, legs, and coccygeal region. These plexuses control the transmission of sensory information and coordinate motor functions of the lower body.
The Lumbar Plexus
The lumbar plexus is situated within the lumbar region of the back and is primarily formed by the first four lumbar spinal nerves (L1 to L4). This plexus extends its branches into several nerves, including the...
2.5K
Nerve Supply of the GI Tract01:27

Nerve Supply of the GI Tract

3.6K
The neuronal supply to the gastrointestinal (GI) tract is essential for regulating various functions, including digestion, absorption, and movement of food. This intricate network of nerves is known as the enteric nervous system (ENS), often referred to as the "second brain" of the body.
The enteric nervous system consists of two major plexuses: the myenteric plexus (Auerbach's plexus) and the submucosal plexus (Meissner's plexus). These plexuses are located within the layers of...
3.6K
Cranial Nerves: Types Part I01:14

Cranial Nerves: Types Part I

5.2K
Cranial nerves are responsible for transmitting motor and sensory information between the brain and various parts of the body. There are twelve pairs of cranial nerves, with the first six being essential in sensory perception, motor control, and autonomic functions related to the head and neck.
Olfactory Nerve (Cranial Nerve I)
The olfactory nerve, or cranial nerve I, is unique as it is purely sensory and dedicated to the sense of smell. This nerve originates in the olfactory epithelium of the...
5.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Coordinated Representational Drift Across the Mouse Cortex.

Research square·2026
Same author

Chronic cortical imaging to measure vibrotactile-response plasticity of deafferented hindlimbs in a rat model of spinal cord injury.

Journal of neuroscience methods·2026
Same author

Coordinated Representational Drift Across the Mouse Cortex.

bioRxiv : the preprint server for biology·2026
Same author

Analysis on optical coherence tomography images to detect irregularities and restoration on paper-based artwork.

Optics express·2025
Same author

Brain-wide neural recordings in mice navigating physical spaces enabled by robotic neural recording headstages.

Nature methods·2024
Same author

Three-dimensional virtual histology of the rat uterus musculature using micro-computed tomography.

Journal of anatomy·2024
Same journal

Fluorescence properties of collagen types I-V: a comprehensive study of spectral and lifetime characteristics.

Journal of biomedical optics·2026
Same journal

Spectral dependence of lipofuscin fluorescence lifetimes revealed by FLIM with a superconducting nanowire single-photon detector.

Journal of biomedical optics·2026
Same journal

Building the future of biophotonics through experiential education and seasonal schools.

Journal of biomedical optics·2026
Same journal

Time-of-flight fluorescence depth mapping using a spatiotemporal deep learning model.

Journal of biomedical optics·2026
Same journal

Erratum: Color-filter-array-based multispectral photoplethysmography optical sensor and its motion artifact correction algorithm (Erratum).

Journal of biomedical optics·2026
Same journal

Real-time fidelity assessment of fluorescence molecular imaging without reference images.

Journal of biomedical optics·2026
See all related articles

Related Experiment Video

Updated: Feb 3, 2026

Doppler Optical Coherence Tomography of Retinal Circulation
10:46

Doppler Optical Coherence Tomography of Retinal Circulation

Published on: September 18, 2012

19.3K

Extracting morphometric information from rat sciatic nerve using optical coherence tomography.

James Hope1,2, Bastian Braeuer1,3, Satya Amirapu4

  • 1The Dodd Walls Centre for Photonic and Quantum Technologies, New Zealand.

Journal of Biomedical Optics
|November 5, 2018
PubMed
Summary
This summary is machine-generated.

Three optical coherence tomography (OCT) techniques were evaluated for peripheral nerve morphometry. While promising for epineurium thickness and fascicle identification, accuracy is limited by tissue scattering.

Keywords:
bio-photonicsmedical and biomedical imagingneurologyoptical coherence tomography

More Related Videos

In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography
07:44

In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography

Published on: July 24, 2020

3.5K
Axoplasm Isolation from Rat Sciatic Nerve
05:29

Axoplasm Isolation from Rat Sciatic Nerve

Published on: September 24, 2010

14.1K

Related Experiment Videos

Last Updated: Feb 3, 2026

Doppler Optical Coherence Tomography of Retinal Circulation
10:46

Doppler Optical Coherence Tomography of Retinal Circulation

Published on: September 18, 2012

19.3K
In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography
07:44

In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography

Published on: July 24, 2020

3.5K
Axoplasm Isolation from Rat Sciatic Nerve
05:29

Axoplasm Isolation from Rat Sciatic Nerve

Published on: September 24, 2010

14.1K

Area of Science:

  • Biomedical Engineering
  • Ophthalmology
  • Neuroscience

Background:

  • Peripheral nerve morphometry is crucial for diagnosing and managing neurological conditions.
  • Optical Coherence Tomography (OCT) offers non-invasive imaging capabilities.
  • Accurate morphometric analysis of peripheral nerves using OCT requires robust image processing techniques.

Purpose of the Study:

  • To evaluate the accuracy of three OCT image analysis techniques for extracting morphometric information from rat peripheral nerves.
  • To compare the performance of Average Depth-Resolved Profile (ADRP), Autoregressive Spectral Estimation (AR-SE), and Correlation of Derivative Spectral Estimation (CoD-SE) against histological measurements.
  • To assess the potential of these OCT techniques for in vivo peripheral nerve characterization.

Main Methods:

  • Three OCT image analysis techniques were applied: ADRP with a scanning window, AR-SE, and CoD-SE.
  • Morphometric measurements, including epineurium thickness and fascicle fiber diameter, were extracted from OCT images.
  • The accuracy of OCT-derived measurements was validated against high-resolution histological data (±1 μm).
  • Statistical analysis (Kruskal-Wallis test) was used to determine agreement between OCT techniques and histology.

Main Results:

  • The ADRP technique, enhanced with a scanning window, improved epineurium thickness estimation, showing agreement with histology in 5/10 images (vs. 2/10 without).
  • The AR-SE technique demonstrated agreement with histology for epineurium thickness in 6/10 images.
  • Both AR-SE and CoD-SE correctly identified fascicles with larger fiber diameters but underestimated the magnitude of the difference (0.5 μm vs. 4 μm actual).
  • Scattering in adipose and neural tissues limited the depth of accurate OCT analysis.

Conclusions:

  • OCT-based morphometric analysis of peripheral nerves shows potential for accurate measurements, particularly for epineurium thickness.
  • The evaluated OCT techniques (ADRP, AR-SE, CoD-SE) offer valuable insights into peripheral nerve structure.
  • Further refinement is needed to overcome depth limitations caused by tissue scattering for comprehensive nerve imaging.